RNA Strand Displacement In Vitro

Nucleic Acid Delivery


Delivery of Plasmid DNA to Mammalian Cells


Delivery of 2'-O-Me RNA to Mammalian Cells

Delivery of Plasmid DNA which transcribes short RNA Inputs

In Vivo RNA Strand Displacement

Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper


Images will go here from April 24th experiment - display red fluorescence in all wells, including those that only got reporter or the wrong input - also see red vesicles indicating reporter comes apart inside the vesicles


Strategy 2: Switch Transfection reagent to RNAiMAX

RNAiMAX is supposed to be a better transfection reagent for double stranded RNA

Images will go here from experiment from June 13th onward where we do not see red vesicles, however we still see whole cell red fluorescence

Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker

Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter

Strategy 4: Nucleofect RNA reporter, RNA inputs

[Strategy 5]: Redesign RNA Reporter