Team:MIT/Results

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iGEM 2012

RNA Strand Displacement for Sensing, Information Processing,
and Actuation in Mammalian Cells

Overview

Placeholder

Biobricks

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Foundational

In Vitro Strand Displacement
Nucleic Acid Delivery
In Vivo Strand Displacement

Sensing

Modeling
In Vitro Sensing

Processing

Modeling
In Vitro Not Gate
Hammerhead Ribozymes

Actuation

Modeling
In Vitro
In Vivo

RNA Strand Displacement In Vitro

Nucleic Acid Delivery

In Vivo RNA Strand Displacement

Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper

Strategy 2: Switch Transfection reagent to RNAiMAX
Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker

Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter

Strategy 4: Nucleofect RNA reporter, RNA inputs

[Strategy 5]: Redesign RNA Reporter

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