Team:TU-Delft/Seq BR4
From 2012.igem.org
Prefix-Promoter(GPD)-BamHI-Kozak-Start-flagtag-gene-Stop-Nde1-Terminator(CYC1)-Suffix
gaattcgcggccgcttctagagCGGTAGGTATTGA
TTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTT
TTTTTAGTTTTAAAACACCAGAACTTAGTTTCGACGGATGGATCCaAaAaA
ATGGACTACAAGGACGACGACGACAAGGAGCGAAGGAACCACAGTGGGAGAGTGAGTGA
ATTTGTGTTGCTGGGTTTCCCAGCTCCTGCCCCACTGCGAGTACTACTATTTTTCCTTTC
TCTTCTGGCCTATGTGTTGGTGTTGACTGAAAACATGCTCATCATTATAGCAATTAGGAA
CCACCCAACCCTCCACAAACCCATGTATACTGACACTCAACTCCATACCCCCATGTACTT
CTTTCTAGCCAACCTCTCCCTTGCAGATGCCTGCTTTGTGTCCACCACAGTCCCTAAGAT
GCTGGCAAACATACAGATCCAGAGTCAGGCCATCTCCTACTCAGGGTGTCTACTACAGTT
GTATTTTTTCATGTTATTTGTGATGCTGGAGGCATTCCTCTTGGCGGTCATGGCCTATGA
CTGCTACGTGGCCATTTGCCACCCACTTCATTACATTCTGATCATGAGCCCTGGGCTCTG
CATCTTCCTCGTGTCTGCATCCTGGATCATGAATGCCCTCCACTCCCTTCTACACACACT
TCTGATGAACAGCCTGTCCTTCTGCGCAAACCATGAGATCCCACACTTCTTCTGTGACAT
CAATCCCCTCCTGAGTCTGTCCTGCACAGACCCCTTCACCAATGAGCTGGTGATCTTCAT
CACTGGGGGTCTCACAGGACTCATTTGTGTGCTTTGCCTGATTATCTCTTACACGAACGT
TTTCTCGACCATCCTGAAGATCCCATCAGCTCAGGGGAAGCGGAAAGCCTTTTCCACCTG
TAGCTCTCATCTCTCCGTGGTCTCTCTCTTCTTTGGGACTTCTTTTTGTGTTGATTTCAG
TTCTCCCTCAACCCACTCGGCCCAGAAGGACACAGTTGCATCAGTGATGTACACAGTGGT
AACTCCAATGTTGAATCCCTTTATCTACAGTTTGTACTGCTTGCGCAACCAAGATGTCAA
AAGAGCGCTACGTCGCACGCTGCACCTGGCCCAGGACCAGGAGGCCAATACCAACAAAGG
CAGCAAAATTGGTTAATAACATATGTCATGTAATTAGTTATGTCACGCTTACATTCACGC
CCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTC
CCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTT
CTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAG
AAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCGGCCtactagtagcggccgctgcag
Total
amount of nt’s: 1463