From 2012.igem.org
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box
| Slot
| Label
| Name
| Part
| Plasmid
| Source
|
| iGEM 2012 Plasmid Box 1
| 2
|
| cadA
|
| ASKA cadA plasmid
| Barrick Lab
|
| iGEM 2012 Plasmid Box 1
| 3
|
| ldcC
|
| ASKA ldcC plasmid
| Barrick Lab
|
| iGEM 2012 Plasmid Box 1
|
| IGM004
| GFP
| [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891]
| pSB3K3
| 2012 kit plate 2 (15L)
|
| iGEM 2012 Plasmid Box 1
|
| IGM005
| RFP
| [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014]
| pSB1AK3
| 2012 kit plate 3 (12O)
|
| iGEM 2012 Plasmid Box 1
|
| IGM006
| LasI
| [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009]
| pSB1A2
| 2012 kit plate 2 (10N)
|
| iGEM 2012 Plasmid Box 1
|
| IGM007
| RBS + LacI
| [http://www.partsregistry.org/Part:BBa_J24679 BBa_J24679]
| pSB1A2
| 2012 kit plate 1 (7L)
|
| iGEM 2012 Plasmid Box 1
|
| IGM008
| Double terminator (BBa_B0010-BBa_B0012)
| [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015]
| pSB1AK3
| 2012 kit plate 1 (23L)
|
| iGEM 2012 Plasmid Box 1
|
| IGM009
| J23100 promoter - sfGFP (Part:BBa_K515105)
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105]
| pSB1C3
| 2012 kit plate 5 (1K)
|
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box
| Slot
| Label
| Description
| Strain
| Source
| Added by
|
| iGEM 2012 Box 1
| 2
| AG1
| Host strain of ASKA collection
|
| Barrick Lab
|
|
| iGEM 2012 Box 1
| 3
| BW25113
| Host strain of Keio collection
|
| Barrick Lab
|
|
| iGEM 2012 Box 1
| 4
| cadA
| AG1 + ASKA collection cadA plasmid, CamR
|
| Barrick Lab
|
|
| iGEM 2012 Box 1
| 5
| ldcC
| AG1 + ASKA collection ldcC plasmid, CamR
|
| Barrick Lab
|
|
| iGEM 2012 Box 1
| 6
| tnaA
| Keio collection knockout (version of odor-less E. coli), KanR
|
| Barrick Lab
|
|
| iGEM 2012 Box 1
| 7
| IGM009
| crdASC (4864 bp)
| TOP10
| Barrick Lab
| Ben
|
| iGEM 2012 Box 1
| 8
| IGM010
| crdASC (4943 bp)
| TOP10
| Barrick Lab
| Ben
|
| iGEM 2012 Box 1
| 9
| IGM011
| crdASC (4864 bp)
| BL21(DE3)
| Barrick Lab
| Ben
|
| iGEM 2012 Box 1
| 10
| IGM012
| crdASC (4943 bp)
| BL21(DE3)
| Barrick Lab
| Ben
|
| iGEM 2012 Box 1
| 11
| IGM013
| BBa_K515105: J23100-sfGFP-pSB1C3(CamR)
| epi300
| Parts Kit
|
|
ZombiE.coli parts
| Plasmid
| Part
| Description
| Source
|
| pReporter
| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
| GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?)
| 2012 kit plate 2 (15L)
|
| pReporter
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
| RFP protein generator - PoPS->RFP
| 2012 kit plate 3 (12O)
|
| pReporter
| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
| LasI protein generator (TERM-)
| 2012 kit plate 2 (10N)
|
| pReporter
| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
| RBS + LacI
| 2012 kit plate 1 (7L)
|
| pReporter
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| Double terminator (BBa_B0010-BBa_B0012)
| 2012 kit plate 1 (23L)
|
| pConverter
| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
| LasR Promoter
| 2012 kit plate 1 (12A)
|
| pConverter
| [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
| LasR
| 2012 kit plate 1 (14J)
|
| pConverter
| [http://partsregistry.org/Part:BBa_K082016 BBa_K082016]
| LacIM1
| Parts Registry
|
| pConverter
| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| LacI Promoter
| 2012 kit plate 1 (1D)
|
| pConverter
|
| CRE
| pQL269 Plasmid
|
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