Team:LMU-Munich/Data/Inducible

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Inducible Bacillus Promoters

Fig. 1: Luminescence measurements of the promoter P_liaI with induction with different concentrations of Bacitracin.

The inducible promoter P_liaI was evaluated in the reporter vector pSB_Bs3C-luxABCDE which contains the lux operon . This is why the promoter activity leads to gene expression and to the production of the protein luciferase. The luminescence of this protein can be measured with the plate reader Synergy2 (Biotek) (Fig.1).

Fig.2: β-galactosidase assay of the inducible promoter _liaI with and without induction with bacitracin

<p align="justify"> The inducible promoter P_liaIwas also evaluated with the reporter vector pSB_Bs1C-lacZ which contains the lacZ reporter gene (Fig.2). Promoter activity leads to the expression of the β-galactosidase which directly correlates to the promoter activity. The β-galactosidase assay of the inducible Bacillus promoter P_liaI was repeated three times. Data show one representative result.