Team:Berkeley/Contributions

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Many of our basic parts (promoters, terminators, RBSs, and fluorescent proteins) are courtesy of the Dueber lab. The iGEM team chose the targeting proteins/sequences from the literature and either PCRed them from genomic DNA or synthesized them, built all of the composite parts, and assayed them.

The Golden Gate assembly of our multi- and multi-multi- gene cassettes was designed and performed by the undergraduate team.

On the software side, the team wrote the MATLAB code and constructed the Cell Profiler pipeline for automated image analysis.