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Team:Trieste/notebook7
From 2012.igem.org
The Jolly JoCare
a safe probiotic platform for protein expression
Week 7
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Suicide System
We received the sequencing results of RBS_B0031-Tse2-TT_B0015 construct and double checked before the BB submission. At this point we were able to clone it downstream the T5LacOperator Promoter (BBa_K875002) to test its functionality. We screened the colonies and two of them resulted positive (T5LacOp-B0031-Tse2-B0015), so we plated them with and without IPTG. One of the two colonies doesn't grow on IPTG and grew without it! We also did the same test in LB culture and the same colony died with IPTG and grew without it. We also recorded the OD of the LB culture IPTG induced and not induced at fixed time intervals for several hours during the day. We designed the growth curve.Antibody
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