Suicide System

We inoculated the positive colonies and also checked by digestion ( EcoRI and XbaI/PstI ). The LL37 fragment, cut XbaI/PstI, was successfully cloned downstream the promoter T5LacOperator cut SpeI/PstI. The M15 cells ( containing the pREP4 plasmid coding for the Lac repressor) were transformed with the construct described above and seeded in Kan ( M15 resistance), Cm ( pSB1C3 resistance) in two conditions non induced and induced with IPTG ( the inhibitors of the repressor Lac).

Unfortunately the LL-37 cathelicidin was probably unable to disrupt the cell membrane, as we observed a growth both in induced and not induced.

The construct T5CumateOperator-LL37 was cut EcoRI/XbaI, heat inactivated at 80∞C for 20í, dephosphorilated at its extremitis.

Antibody

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Chassis

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.