Team:SDU-Denmark/labwork/Notebook/week4

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iGEM TEAM ::: SDU-DENMARK

Laboratory Notebook

Here you find the log book for the procedures carried out in the laboratory, starting from week 27.

1st week	2nd week	3th week	4th week
5th week	6th week	7th week	8th week

23-07-2012:

SST send for sequencing

SST from the gel extraction the 21-07-2012 was diluted to a concentration of 100ng/µL and separated to 4 samples of 15µL each and send of for sequencing.
We made liquid cultures from the SST plates from 19-07-2012 and incubated them O.N.

24-07-2012:

Cryo backup, miniprep and preparation to sequencing

We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on.
We ran a gel on the samples and found that three samples had bands corresponding to our cut gene and vector. These will be shipped of to be sequneced tomorrow.

24-07-2012:

SST send for sequencing

Dato 25/7-12 Kristian og Lars Sample 2.3 and 2.6 was prepared for sequencing by diluting them 6:15 giving a total of around 70-78 ng/uL and sent them for sequencing. Afterward we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing. Lars will upload the work when it is done (between now and the weekend). -results from sequencing -primers ordered