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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
Revision as of 21:05, 18 June 2012 by Erik.quandt (Talk | contribs)
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Slot | Label | Name | Part | Plasmid | Source |
|---|---|---|---|---|---|---|
| iGEM 2012 Plasmid Box 1 | 2 | cadA | ASKA cadA plasmid | Barrick Lab | ||
| iGEM 2012 Plasmid Box 1 | 3 | ldcC | ASKA ldcC plasmid | Barrick Lab | ||
| iGEM 2012 Plasmid Box 1 | IGM004 | GFP | [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891] | pSB3K3 | 2012 kit plate 2 (15L) | |
| iGEM 2012 Plasmid Box 1 | IGM005 | RFP | [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014] | pSB1AK3 | 2012 kit plate 3 (12O) | |
| iGEM 2012 Plasmid Box 1 | IGM006 | LasI | [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009] | pSB1A2 | 2012 kit plate 2 (10N) | |
| iGEM 2012 Plasmid Box 1 | IGM007 | RBS + LacI | [http://www.partsregistry.org/Part:BBa_J24679 BBa_J24679] | pSB1A2 | 2012 kit plate 1 (7L) | |
| iGEM 2012 Plasmid Box 1 | IGM008 | Double terminator (BBa_B0010-BBa_B0012) | [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015] | pSB1AK3 | 2012 kit plate 1 (23L) |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Name | Description | Source | |
|---|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab | |
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab | |
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab | |
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab | |
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab | |
| iGEM 2012 Box 1 | 7 | IGM009 | crdASC (4864 bp) in TOP10 | Barrick Lab | |
| iGEM 2012 Box 1 | 8 | IGM010 | crdASC (4943 bp) in TOP10 | Barrick Lab | |
| iGEM 2012 Box 1 | 9 | IGM011 | crdASC (4864 bp) in BL21(DE3) | Barrick Lab | |
| iGEM 2012 Box 1 | 10 | IGM012 | crdASC (4943 bp) in BL21(DE3) | Barrick Lab | |
| iGEM 2012 Box 1 | 11 | IGM013 | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) in epi300 | Parts Kit |
ZombiE.coli parts
| Plasmid | Part | Description | Source |
|---|---|---|---|
| pReporter | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | 2012 kit plate 2 (15L) |
| pReporter | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | RFP protein generator - PoPS->RFP | 2012 kit plate 3 (12O) |
| pReporter | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | LasI protein generator (TERM-) | 2012 kit plate 2 (10N) |
| pReporter | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS + LacI | 2012 kit plate 1 (7L) |
| pReporter | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator (BBa_B0010-BBa_B0012) | 2012 kit plate 1 (23L) |
| pConverter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | LasR Promoter | 2012 kit plate 1 (12A) |
| pConverter | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | LasR | 2012 kit plate 1 (14J) |
| pConverter | [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] | LacIM1 | Parts Registry |
| pConverter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | LacI Promoter | 2012 kit plate 1 (1D) |
| pConverter | CRE | pQL269 Plasmid |
