1. Download the following spreadsheet : File:Team-EPF-Lausanne Ligation.xls

2. Fill in the pink areas with the vector and fragment concentration, their size and the ratio.

3. Add all the suggested ingradients in a microcentrifuge tube, in the order they appear.

4. Ligate for 2 hours at 14ºC.

5. Inmediatly transform competent bacteria with the ligation product.