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Protocol: Ligation
- Download the following spreadsheet : File:Team-EPF-Lausanne Ligation.xls
- Fill in the pink areas with the vector and fragment concentration, their size and the ratio.
- Add all the suggested ingradients in a microcentrifuge tube, in the order they appear.
- Ligate for 2 hours at 14ºC.
- Inmediatly transform competent bacteria with the ligation product.
