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Contents 1 Organisms 1.1 Organisms used in our LabWork 1.1.1 Escherichia coli 1.1.2 Saccharomyces cerevisiae 1.2 Organisms from which genes were derived 1.2.1 Sulfolobus acileratius 1.2.2 Gibberella fujikuroi

Organisms

Organisms used in our LabWork

Escherichia coli

E.coli is a well known model organism for molecular biology. It is a gram negative bacteria whose generation time is about 30 minutes. We used E.coli to amplify the plasmids that we isolated from yeast after transformation via homologue recombination.

Saccharomyces cerevisiae

S.cerevisiae is our main organism. We want to produce steviol in yeast and we did the transformations in this organism. In addition we are able to do the transformations via gap repair which is possible in yeast because of its ability for homologue recombination with only about 40 bp. Furthermore S.cerevisiae has got an ER which is needed because some enzymes for steviol production are localized in the ER, S.cerevisiae is an established food additive producer and is closer related to Stevia than E.coli for example.

Organisms from which genes were derived

Sulfolobus acileratius

We used the GGPP synthase from this organism.

Gibberella fujikuroi

We used the kaurene oxidase and the bifunctional cyclase from this organism.