Team:Macquarie Australia/Protocols/ligations

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Ligation Procedure

A reaction mixture was prepared containing,

Destination Plasmid</td> <td>2 µL</td> <tr><td>10X T4 DNA ligase buffer</td> <td>2 µL</tr></td> <tr><td>T4 DNA ligase</td> <td>1 µL</td></tr> <tr><td>H2O</td> <td>11 µL</td></tr> </table></center> </html>
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Element Volume
Upstream Digestion part 2 µL
Downstream Digestion part 2 µL