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Team:Kyoto/GoldenGateAssembly/Notebook
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Contents |
Golden Gate Assembly Notebook
August 10
Golden Gate Assembly method This method helps us to constract some genes quickly and we can design the order of constractions. We are trying to construct a gene cycle composed of 6 genes( pSB1A3, lacI, GFP, RFP, GFP, DT). After this experiment is confirmed to work, we will so some experiment to check how the numbers of promorters influences the strength of transcription.
September 6
PCR
| Quick Taq | primer F | primer R | DNA | MilliQ | Total |
|---|---|---|---|---|---|
| 12.5 | 0.5 | 0.5 | 1 | 10.5 | 25 |
