Primers Digestion Transformation

All primers are notated 5' to 3'.

Please refer to the Primer annealing lab book entry for further detail.

For primer annealing in the PCR, the primer sequences were combined in the following way:

Please refer to the Primer annealing lab book entry for further detail.

pGEX-6P-1 was cut using EcoR1(954) and Xho1 (975)

pGEX vector with insert transformed into DH5α E. coli cells to replicate.

pGEX vector with G-Block insert transformed into DH5α E. coli cells to replicate.

pGEX vector with insert transformed into BL21 E. coli cells for protein expression.

pGEX vector with G-Block insert transformed into BL21 E. coli cells for protein expression.