Team:Exeter/lab book/proto/5

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Protocol 5


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase

Annealing Oligomer Primers
Add to each primer tube annealing buffer calculated to achieve 200uM. Mix 25ul of each appropriate oligomer pair together in a PCR tube . Heat at 95° for two minutes then cool to 30° over an hour by decreasing by 0.5°C per thirty second cycle for the 130 cycles. Cool rapidly to 4° or place in the freezer.