Western Blot result from 8.sep.12

• Lane 1: Ladder
• Lane 2: 10(or 30) Lovtap Sample 1(100% lovtap)
• Lane 3: 10(or 30) Lovtap Sample 2(100% lovtap)�
• Lane 4: 10(or 30) Lovtap Sample 3(50% lovtap+50% filler)�
• Lane 5: 10(or 30) Lovtap Sample 4(50% lovtap+50% filler)�
• Lane 6: 20(or 40) Lovtap Sample 1(100% lovtap)�
• Lane 7: 20(or 40) Lovtap Sample 2(100% lovtap) �
• Lane 8: 20(or 40) Lovtap Sample 3(50% lovtap+50% filler)
• Lane 9: 20(or 40) Lovtap Sample 4(50% lovtap+50% filler)�
• Lane 10: 20(or 40) non-transfected CHO cell as a control + 5microG of VP16 only for the first membrane

Membrane was dirt by wrong secondary antibody and not washed well.

Cannot confirm the Lovtap-vp16 expression.

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