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Miniprep psb1-c3
minipreps of biobrick plasmid containing rfp were done.
Overnight cultures of psb1-c3-rfp, psb1-c3 -lovTAP and pcep4-HA-readout were prepared.
Western Blot result from 5.sep.12
- Double check VP16 antibody detection with 4.sep.12's result
Protocol: Miniprep
The slim tubes can be centrifuged in the machine in front of the "Gel hood", at 4000 rpm for 10 min. The fatter ones, in the E. coli centrifuge by the fridge (the tip can be left inside, since it floats).
Pellets resuspended with RNase containing buffer (Resuspension Buffer R3, from Invitrogen, equivalent to Buffer P1 from Qiagen, in Sowmya's box in the fridge). Note: keep the buffer in ice if you are not bringing it back to the fridge for some minutes.
We then use the QIAGEN QIAprep Spin Miniprep Kit with their [http://www.qiagen.com/literature/render.aspx?id=370 protocol] (page 22) and a microcentrifuge.
- Comments
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