"

From 2012.igem.org

Welcome to the NRP UEA iGEM 2012 Wiki Projects Menu

Please choose the relevant link to view an overview of each project!

Nitric Oxide Sensing & The Hybrid Promoters | The Comparator Circuit | Theoretical Projects

SUMMARY OF IT ALL HERE; similar to front page

Contents 1 Origins of the Idea 2 Preliminary Lab 3 Lab Work 4 Results 5 Conclusions 6 Future Applications 7 Future Experiments

Origins of the Idea

. UEA uses NO

. Difficult to sense (machines and biologically)

. Important applications e.g. cancer and buildings so useful to work it out

Preliminary Lab

. Looked at PyeaR and CArG, ligated them together in two orientations for a flexible promoter (info on what each is)

Lab Work

. Made into biobricks (link to registry page)

. Ligated to RFP/CFP and got photos to prove it works

. Used RFP/CFP with the fluorometer etc. to get data on sensitivity

. Tranfected into mammalian cells to show flexibility

. Studied the growth rate

Results

. Graphs from flow and fluorometer

. Pictures etc. from mammalian transfection

. Growth study on MB/BM

Conclusions

BM/MB better or the same, RFP/CFP better or the same etc.

Future Applications

. Cancer

. Buildings

. Soil

Future Experiments

. With different substrates (e.g. nitrite salt, NO donor)

. With different reporter/effector enzyme