Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box	Slot	Name	Description	Source
iGEM 2012 Plasmid Box 1	2	cadA	ASKA cadA plasmid	Barrick Lab
iGEM 2012 Plasmid Box 1	3	ldcC	ASKA ldcC plasmid	Barrick Lab

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box	Slot	Name	Description	Source
iGEM 2012 Box 1	2	AG1	Host strain of ASKA collection	Barrick Lab
iGEM 2012 Box 1	3	BW25113	Host strain of Keio collection	Barrick Lab
iGEM 2012 Box 1	4	cadA	AG1 + ASKA collection cadA plasmid, CamR	Barrick Lab
iGEM 2012 Box 1	5	ldcC	AG1 + ASKA collection ldcC plasmid, CamR	Barrick Lab
iGEM 2012 Box 1	6	tnaA	Keio collection knockout (version of odor-less E. coli), KanR	Barrick Lab

Plasmid	Part	Description	Source
pReporter	[http://partsregistry.org/Part:BBa_I714891 BBa_I714891]	GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?)	2012 kit plate 2 (15L)
pReporter	[http://partsregistry.org/Part:BBa_J06504 BBa_J06504]	Monomeric RFP optimized for bacteria	2012 kit plate 1 (13F)
pReporter	[http://partsregistry.org/Part:BBa_K081009 BBa_K081009]	LasI protein generator (TERM-)	2012 kit plate 2 (10N)
pReporter	[http://partsregistry.org/Part:BBa_J24679 BBa_J24679]	RBS + LacI	2012 kit plate 1 (7L)
pReporter	[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]	Double terminator (BBa_B0010-BBa_B0012)	2012 kit plate 1 (23L)
pConverter	[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]	LasR Promoter	2012 kit plate 1 (12A)
pConverter	[http://partsregistry.org/Part:BBa_C0079 BBa_C0079]	LasR	2012 kit plate 1 (14J)
pConverter	[http://partsregistry.org/Part:BBa_K082016 BBa_K082016]	LacIM1	Parts Registry
pConverter	[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]	LacI Promoter	2012 kit plate 1 (1D)
pConverter		CRE	pQL269 Plasmid