Recipes

• Preparation of LB Medium
• Competent Cell Preparation
• TAE Production
• Agar Plates Preparation

Cloning

• PCR
• Gel Electrophoresis
• Restriction Site Digestion
• Quick and Dirty Digestion
• Ligation
• E.coli Transformation
• Prepare Plasmid Extraction
• Miniprep

Mammalian

• CHO Transfection
• HEK Transfection
• Trypan Blue Method

Expression

• Western Blot
• Flowcytometry (Guava)
• Sampling for Tecan machine

DNA Quality

• DNA Concentration Measurement
• DNA Sequencing Sample Preparation