Team:Macquarie Australia/Protocols/GibsonAssembly2

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3A 3K 3C 4A 4K 4C 5A 5K 5C
1.addition of 20ng gene fragments in appropriate tube 2μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E 2μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E 2μl each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E 2μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E 2μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E 2μl each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E 2μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E 2μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E 2μl each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E
2. Addition of 0.05pmol of vector 2.8µl PSB-1A3 2.9µl PSB-1K3 2.7µl PSB-1C3 2.8µl PSB-1A3 2.9µl PSB-1K3 2.7µl PSB-1C3 2.8µl PSB-1A3 2.9µl PSB-1K3 2.7µl PSB-1C3
3. Addition of Master Mix (μl) 12.8 12.9 12.7 12.8 12.9 12.7 12.8 12.9 12.7