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Team:Tec-Monterrey/antifreeze/project
From 2012.igem.org
This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetlle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.
We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the neccesary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.
When Escherechia Ecolis are stored,water molecules create crystals that may harm the bacteria.
To avoid this, we place our plasmid inside E.coli
This plasmid produces antifreeze proteins that travel from inside the E.coli towards its membrane and medium.
These proteins will avoid the formation of larger crystals inside E.coli, its membrane and the medium.
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This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetlle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells. We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the neccesary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation. |
