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Team:Tec-Monterrey/allergen/project
From 2012.igem.org
Our project is based in the development of a standard production and expression of allergen proteins (from maize, dustmite and bees) in order for them to be easily purified and used in a new diagnostic kit designed for the detection of allergies.
The project involves the construction of device that would allow the expression of allergen proteins in yeast, resulting in the secretion of the proteins to the medium, while also maintaining the ability to also produce the proteins in E. coli. The design of the kit is based on an ELISA assay and includes the production of modified scFv’s (single-chain variable fragment) that allow the detection of possible allergic reactions in an individual.
We place our plasmid inside P.pastoris.
Once inside, it produces one of 5 desired proteins.
This protein is then taken to membrane and sent to the medium.
With a centrifuge process P.pastoris is removed.
All other elements are removed during purification.
We then place our proteins inside coated microtubes.
A patient's blood sample is placed inside each microtube.
If the patient is allergic, the microtube will glow.
This system is designed to identify corn, dust mite and bee venom allergies.
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PS: step up son
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Our project is based in the development of a standard production and expression of allergen proteins (from maize, dustmite and bees) in order for them to be easily purified and used in a new diagnostic kit designed for the detection of allergies. The project involves the construction of device that would allow the expression of allergen proteins in yeast, resulting in the secretion of the proteins to the medium, while also maintaining the ability to also produce the proteins in E. coli. The design of the kit is based on an ELISA assay and includes the production of modified scFv’s (single-chain variable fragment) that allow the detection of possible allergic reactions in an individual. |
