Team:Carnegie Mellon/Bio-Submitted

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Submitted Parts

BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: CAATACGACTCACTATAGGGCAATTGTGAGCGCTCACAATTGCAA
Mutant III: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant IV: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Hypothesis:

Expected promoter strength of different mutants:
Mutant I: <100%
Mutant II: >>100%
Mutant III: ~100%
Mutant IV: ~50%

Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Below average
Mutant III: Average
Mutant IV: Average

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