"
Team:Penn/Notebook
From 2012.igem.org
June 2012 Notebook
Week 1
June 6th
- Set up some lab equipment
- Autoclaved for a while
- Organized biobrick stuff
- Called Vinoo about DNA planning
June 7th
- Transformed Cph8, pLsr, and LuxS
- Placed order with Vinoo
- Developed idea using PGY/PCN system to activate a gene
Week 2
June 11th
Wet Lab
- PCR'd mCherry from NAS157
- Ran 1% Gel and purified product
Dry Lab
- Designed primers for LsR promoter
- Meeting with Dr. Sarkar
June 12th
Wet Lab
- Digested mCherry PCR product with BamHI and NotI
- Column purified mCherry and ligated into NAS152 backbone
- Transformed NAS152-mCherry into DH5alpha
- Poured 25 LB-Kan plates
Dry Lab
- Research more information about bacterial drug delivery system
- More research into biofilm project
June 14th
Wet Lab
- Fill in later....
Dry Lab
- Met with Dr. Goulian, obtained pDawn and pDusk
- Identified inaK as a surface display gene we can use
Week 3
June 18th
Wet Lab
- Miniprep pDawn and pDusk
- Test cut pDawn and pDusk with XmaI, analytical gel was correct
- Prep cut pDawn and pDusk with BamHI and NotI, gel purified
Dry Lab
- Ordered and picked up PCR purification kit from cell center
- Additional orders through cell center
- Designed primers for one of Peter's components (forgot which)
June 20
Wet Lab
- Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
- PCR purified fragments (Peter), then ran gel?
Dry Lab
- Researched DARPin binding domains and linkers
- Finalized some biobrick orders
- Finalized synthesis order (minus linker)
Week 1
June 6th
- Set up some lab equipment
- Autoclaved for a while
- Organized biobrick stuff
- Called Vinoo about DNA planning
June 7th
- Transformed Cph8, pLsr, and LuxS
- Placed order with Vinoo
- Developed idea using PGY/PCN system to activate a gene
Week 2
June 11th
Wet Lab
- PCR'd mCherry from NAS157
- Ran 1% Gel and purified product
Dry Lab
- Designed primers for LsR promoter
- Meeting with Dr. Sarkar
June 12th
Wet Lab
- Digested mCherry PCR product with BamHI and NotI
- Column purified mCherry and ligated into NAS152 backbone
- Transformed NAS152-mCherry into DH5alpha
- Poured 25 LB-Kan plates
Dry Lab
- Research more information about bacterial drug delivery system
- More research into biofilm project
June 14th
Wet Lab
- Fill in later....
Dry Lab
- Met with Dr. Goulian, obtained pDawn and pDusk
- Identified inaK as a surface display gene we can use
Week 3
June 18th
Wet Lab
- Miniprep pDawn and pDusk
- Test cut pDawn and pDusk with XmaI, analytical gel was correct
- Prep cut pDawn and pDusk with BamHI and NotI, gel purified
Dry Lab
- Ordered and picked up PCR purification kit from cell center
- Additional orders through cell center
- Designed primers for one of Peter's components (forgot which)
June 20
Wet Lab
- Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
- PCR purified fragments (Peter), then ran gel?
Dry Lab
- Researched DARPin binding domains and linkers
- Finalized some biobrick orders
- Finalized synthesis order (minus linker)
Retrieved from "http://2012.igem.org/Team:Penn/Notebook"
