Team:HokkaidoU Japan/Notebook/plastic Week 6

polymer extract

@Taguchi lab

1. sample1 JM109 culture from July18th 1.4643g , 0.0054 (tube+polymer , polymer)
2. sample2 JM109 culture from July18th 1.4642g , 0.0040
3. sample3 DH5a 1.4666g , 0.0056

preculture

Inoculated from three different plates(Name it 1,2,3)
Cultured in media of LB 150microL+Amp1.5microL
30℃ 20:00-7th10:00

liquid culture

We made 8sapmles to see if Panthotenic acid is nessesary for plastic growth.
Panthotenic acid (+)1.5ml 4samples
Panthotenic acid (+)0.7ml 2samples
Panthotenic acid (-)0.7ml 2samples
Started liquid culture from Aug 7th 11:00 until Aug 9th 09:50.</ br>

@Taguchi lab

Harvest

We did the protocol of Harvest on 8samples.

miniprep

BBa_K342001(PhaC)was minipreped by FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions.

DNA sequencing

BBa_K342001(PhaC) was seqenced.