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Team:Cambridge/Protocols/GetSpores
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Producing high yields of B.subtilis spores
Preparation
Plate and grow B. subtilis overnight on antibiotic resistant plates.
Method
- Pick a sing colony from a new plate of B. subtilis and use to inoculate 50ml CDSM.
- Grow this for 11 hours in a 37oC incubator with vigorous aeration.
- Transfer 2-5% of this to make up another 50ml volume of CDSM culture and grow both for a total incubation time of 48 hours.
- Longer incubation may be needed for full release of spores.
