From 2012.igem.org
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
Box
| Slot
| Name
| Description
| Source
|
iGEM 2012 Plasmid Box 1
| 2
| cadA
| ASKA cadA plasmid
| Barrick Lab
|
iGEM 2012 Plasmid Box 1
| 3
| ldcC
| ASKA ldcC plasmid
| Barrick Lab
|
Strain Database
All strains are in the back right rack of the –80°C freezer.
Box
| Slot
| Name
| Description
| Source
|
iGEM 2012 Box 1
| 2
| AG1
| Host strain of ASKA collection
| Barrick Lab
|
iGEM 2012 Box 1
| 3
| BW25113
| Host strain of Keio collection
| Barrick Lab
|
iGEM 2012 Box 1
| 4
| cadA
| AG1 + ASKA collection cadA plasmid, CamR
| Barrick Lab
|
iGEM 2012 Box 1
| 5
| ldcC
| AG1 + ASKA collection ldcC plasmid, CamR
| Barrick Lab
|
iGEM 2012 Box 1
| 6
| tnaA
| Keio collection knockout (version of odor-less E. coli), KanR
| Barrick Lab
|
ZombiE.coli parts
Plasmid
| Part
| Description
| Sequence
| Source
|
pReporter
| BBa_I714891
| GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?)
|
| 2012 kit plate 2 (15L)
|
pReporter
| BBa_J06504
| Monomeric RFP optimized for bacteria
|
| 2012 kit plate 1 (13F)
|
pReporter
| BBa_K081009
| LasI protein generator (TERM-)
|
| 2012 kit plate 2 (10N)
|