Team:Bielefeld-Germany/Labjournal/week3

Revision as of 13:24, 13 September 2012 by Juvoss (Talk | contribs)

Week 3 (05/14 - 05/20/12)

weekly seminar:

first lab service: Robert
our GFP, which we wanted to use for the summer school for pupils, does not work
first competent cells have to be made: Julia S. and Robert
Decision to buy a commercial laccase to establish the analytics and the enzyme tests
our expose has to be translated into english: Malak
last planning for our waver sell
Julia S. is creating a vector for Pichia pastoris and is now looking for sequences
The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process

Team Activity Tests: For some pre test and characterization for our future laccase activity standard we ordered [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de&region=DE laccase] from Trametes versicolor. As well we had to order a substrate that the laccase could use to demonstrate its abilities. According to the literature [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de&region=DE ABTS] is a well working substrate to characterize oxidizing enzym activity. So we ordered.

Team Bacterial Laccases: For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.

Team Modeling: Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
Team Bacterial Laccases: Plasmid isolation of BBa_J04450 (pSB1C3 + RFP).

Sunday