Team:EPF-Lausanne/Protocol/PCR

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Protocol: PCR

1X Mastermix 20μl reaction

Reagent	Volume [μl]
Water	20-x
HF-Buffer (5x)	4
DMSO (optional)	0.6
dNTPs	0.4
Forward primer (50μM)	0.2
Reverse primer (50μM)	0.2
Template (10ng/μl)	0.5
Phusion HF polymerase	0.2

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