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Protocol: PCR
1X Mastermix 20μl reaction
20μl - x
- DMSO (optional) 0.6μl
- dNTPs 0.4μl
- Primers (50μM)
- Forward 0.2μl
- Reverse 0.2 μl
- Template (10ng/μl) 0.5μl
- Phusion HF polymerase 0.2μl
| Reagent | [μl] |
|---|---|
| Water | 20-x |
| HF-Buffer (5x) | 4 |
| DMSO (optional) | 0.6 |
| dNTPs | 0.4 |
| Forward primer (50μM) | 0.2 |
| Reverse primer (50μM) | 0.2 |
| Template (10ng/μl) | 0.5 |
| Phusion HF polymerase | 0.2 |
