Team:TU Darmstadt/Labjournal/Metabolism

From 2012.igem.org

Revision as of 09:16, 9 September 2012 by SaHein (Talk | contribs)

Contents

week 1 (14.-18.05.12)

tphA1

  • No work progress

tphA2

  • No work progress

tphA3

  • No work progress

tphB

  • No work progress

aroY

  • No work progress

Other

  • Reconstitution of C. testosteroni KF-1 according to DSMZ [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf protocol]
  • Cultivation of C. testosteroni KF-1 on agar plates with Medium 1
  • Production of chemically competent E. coli DH5α and E. coli BL21(DE3)pLysS cells

week 2 (21.-25.05.12)

tphA1

  • Isolation of the gene from C. testosteroni KF-1 genome using colony-PCR
    • Annealing temperature: 49 °C
    • Primer: tphA1-l-F and tphA1-l-R

tphA2

  • No work progress

tphA3

  • Isolation of the gene from C. testosteroni KF-1 genome using colony-PCR
    • Annealing temperature: 59 °C
    • Primer: tphA3-l-F and tphA3-l-R

tphB

  • Isolation of the gene from C. testosteroni KF-1 genome using colony-PCR
    • Annealing temperature: 59 °C
    • Primer: tphB-l-F and tphB-l-R

aroY

  • No work progress

Other

Biobrick Concentraion [ng/µl]
BBa_K316003 -
BBa_J23100 -
BBa_B0015 -
BBa_J61101 -

week 3 (28.05.-01.06.12)

tphA1

  • PCR on tphA1 isolated from C. tesosteroni
    • Annealing temperature: 69 °C
    • Primer: tphA1-l-PstI(99)-R and tphA1-l-R
  • PCR on tphA1 isolated from C. tesosteroni
    • Annealing temperature: 69 °C
    • Primer: tphA1-l-PstI(99)-F and tphA1-l-F

tphA2

  • No work progress

tphA3

  • No work progress

tphB

  • No work progress

aroY

  • No work progress

Other

week 4 (04.-08.06.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 5 (11.-15-06.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 6 (18.-22.06.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 7 (25.-29.06.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 8 (02.-06.07.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 9 (09.-13.07.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 10 (16.-20.07.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 11 (23.-27.07.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 12 (30.07.-03.08.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 13 (06.-10.08.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 14 (13.-17.08.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 15 (20.-24.08.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 16 (27.-31.08.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 17 (03.-07.09.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 18 (10.-17.09.12)

tphA1

tphA2

tphA3

tphB

aroY

Other

week 19 (17.-21.09.12)

tphA1

tphA2

tphA3

tphB

aroY

Other