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Team:Duke
From 2012.igem.org
Team Biosafety
Designing the modern optogenetic toolkit: from principle to practice
Characterization of rapidly perturbable optogentic logic through a proof-of-concept in yeast cell cycle control
/// The Team and Our Pursuit
Thorough characterization of key genes and controlling cellular oscillation precisely.
Hard Work and Dedication
Our team is fully aware of it's disadvantages, being smaller than the rest, and holding less funding than the competition. However, these notions motivate each individual on the team rather than discourage us. We know that we will get out of this project, exactly what we put into it. Understanding this notion is why it is not unusual to find our team working 12 hour shifts daily. We are inspired and determined to contribute to the scientific community in a substantial way, utilizing our resources, and setting new standards.
Optogenetics, the HOT TOPIC
Optogenetics is the combination of genetic and optical methods to control specific events in targeted cell. In 2010, optogenetics was chosen as the Method of the Year across all fields of science and engineering by the interdisciplinary research journal Nature Methods. At the same time, optogenetics was highlighted in the article on "Breakthroughs of the Decade" in the scientific research journal Science Breakthrough of the Decade.
/// Check out our Lab Journal and Notes
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Preparative Digestion and Electrophoresis
JOURNAL ENTRY by Morgan J. Howell on June 22, 2012
Today I attempted to ligate the insert mKate with the vector YFP. I intially digested mKate and YFP using the digestive enzymes BAMHI-HF and ASC-I, with Buffer #4. After the one and a half hour digestion, I prepared a deep well gel and inserted the digested plasmids tagged with orange dye. After the run, I took the gel into a dark room, and using a black light table and sterile razor blade ...
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Anoter blog post
Fantasy by Lewis Carroll on Aug 24, 2011
It is a long established fact that a reader will be distracted by the readable content of a page when looking at its layout. The point of using Lorem Ipsum is that it has a more-or-less normal distribution of letters, as opposed to using 'Content here, content here'.
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Anoter blog post
Fantasy by Lewis Carroll on Aug 24, 2011
It is a long established fact that a reader will be distracted by the readable content of a page when looking at its layout. The point of using Lorem Ipsum is that it has a more-or-less normal distribution of letters, as opposed to using 'Content here, content here'.
Read More -
Anoter blog post
Fantasy by Lewis Carroll on Aug 24, 2011
It is a long established fact that a reader will be distracted by the readable content of a page when looking at its layout. The point of using Lorem Ipsum is that it has a more-or-less normal distribution of letters, as opposed to using 'Content here, content here'.
Read More
