From 2012.igem.org

Digestion protocol

- 8 uL DNA linearized plasmid Backbone (25 ng/uL)

- 1uL PstI

- 1uL EcoRI

- 2.5 uL H Buffer

- X uL Water

TOTAL 25 uL

-- The digestion mixture is kept for 3 hours at 37 ° C

-- The mixture is kept for 20 minutes at 80ºC

After those 20 minutes, the plasmid pSB1C3 is ready to use.