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User contributions
From 2012.igem.org
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- 08:23, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 08:23, 3 October 2012 (diff | hist) N File:Washington Concentration Gradient.png (top)
- 08:22, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 05:19, 3 October 2012 (diff | hist) N Team:Washington/Protocols/Elect. (Created page with "{{Template:Team:Washington/Templates/Top}} =Electroporation Protocol= # Obtain a competent cell aliquot from the -80oC freezer, keep the tubes on ice. # Add 40μL of ice cold...") (top)
- 05:19, 3 October 2012 (diff | hist) m Team:Washington/Protocols
- 05:05, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 05:01, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 04:57, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 04:56, 3 October 2012 (diff | hist) Team:Washington/Optogenetics (→E.colight app feature list:)
- 04:50, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 03:09, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 02:55, 3 October 2012 (diff | hist) Team:Washington/Optogenetics
- 00:39, 3 October 2012 (diff | hist) Team:Washington/Outreach (top)
- 00:27, 3 October 2012 (diff | hist) Team:Washington/Flu
- 00:26, 3 October 2012 (diff | hist) Team:Washington/Flu
- 10:28, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 10:26, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 10:25, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 10:23, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 10:21, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 09:59, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 09:42, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 09:41, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 09:40, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 09:37, 2 October 2012 (diff | hist) Team:Washington/Optogenetics
- 00:04, 2 October 2012 (diff | hist) N File:Washington rashmi ravichandran.jpg
- 07:08, 29 September 2012 (diff | hist) Team:Washington/Outreach
- 07:03, 29 September 2012 (diff | hist) File:String Theory.png (uploaded a new version of "File:String Theory.png") (top)
- 06:45, 29 September 2012 (diff | hist) Team:Washington/Outreach
- 06:44, 29 September 2012 (diff | hist) Team:Washington/Outreach
- 06:43, 29 September 2012 (diff | hist) Team:Washington/Outreach
- 06:43, 29 September 2012 (diff | hist) N File:String Theory.png
- 06:42, 29 September 2012 (diff | hist) Team:Washington/Outreach
- 03:33, 29 September 2012 (diff | hist) N File:Washington benjamin groves.jpg (top)
- 22:03, 21 September 2012 (diff | hist) Team:Washington/Optogenetics
- 22:03, 21 September 2012 (diff | hist) N File:AppQRCode.jpg (top)
- 22:02, 21 September 2012 (diff | hist) Team:Washington/Optogenetics
- 23:29, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Glycerol Stocks (Created page with "{{Template:Team:Washington/Templates/Top}} =Glycerol Stocks= For the long-term storage of microbial cultures ''Make sure that you do not contaminate glycerol stocks'' 1. Gr...") (top)
- 23:29, 17 September 2012 (diff | hist) N Team:Washington/Protocols/CompDesign (Created page with "{{Template:Team:Washington/Templates/Top}} =center= As can be seen below Foldit uses an easily learned user interface and us...") (top)
- 23:29, 17 September 2012 (diff | hist) N Team:Washington/Protocols/sequencing (Created page with "{{Template:Team:Washington/Templates/Top}} =DNA Sequencing= # Add 10uL of miniprep DNA to an PCR Strip Tubes. The only labels on the Tube should be JS01 ... JSXX (depending o...") (top)
- 23:28, 17 September 2012 (diff | hist) N Team:Washington/Protocols/gene assembly (Created page with "{{Template:Team:Washington/Templates/Top}} =Gene Synthesis= #Generate Oligo's ##Go to: http://helixweb.nih.gov/dnaworks/ ##Set parameters ###Enter you job title and email ###Ch...")
- 23:28, 17 September 2012 (diff | hist) N Team:Washington/Protocols/expression purification (Created page with "{{Template:Team:Washington/Templates/Top}} ='''Protein Expression/Purification of Recombinant Protein in pET29b+ Using Autoinduction'''= __TOC__ Adopted from [http://www.scien...") (top)
- 23:27, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Kunkel (Created page with "{{Template:Team:Washington/Templates/Top}} ='''Kunkel Mutagenesis'''= __TOC__ =='''Produce ssDNA (RECOMMENDED TO DO IN DUPLICATE! SEE NOTE AT END OF SECTION 2) '''== *Transfo...") (top)
- 21:15, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Competent (Created page with "{{Template:Team:Washington/Templates/Top}} =Competent Cell Stocks= '''CCMB Transformation''' 1. Plate 5ul of cells onto an antibiotic free LB/Agar plate. 2. Incubate over...") (top)
- 21:15, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Colony (Created page with "{{Template:Team:Washington/Templates/Top}} =Colony PCR with Green tag= Master mix (7ul): 1ul 10uM forward primer 1ul 10uM reverse Primer 5ul 2x Green tag Cell water (3ul):...") (top)
- 21:14, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Transformation (Created page with "{{Template:Team:Washington/Templates/Top}} =General Transformation Protocol= #Thaw 20 μl of frozen competent cells on ice. Do not use glass tubes, which adsorb DNA. #Add 2...") (top)
- 21:13, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Ligation (Created page with "{{Template:Team:Washington/Templates/Top}} =General Ligation Protocol= 7uL insert 1uL vector 1uL T4 ligase buffer 1uL T4 ligase Incubate at 16C overnight, or room temperat...") (top)
- 21:12, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Digestion (Created page with "{{Template:Team:Washington/Templates/Top}} =General Digestion Protocol= 10 uL DNA 5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of en...")
- 21:11, 17 September 2012 (diff | hist) N Team:Washington/Protocols/PCR (Created page with "{{Template:Team:Washington/Templates/Top}} =General PCR Protocol= #Setup Amplification PCR Reaction ##1uL Template ##1uL 25mM dNTP's ##10uL Phusion HF Buffer ##0.5uL Forward P...")
- 21:04, 17 September 2012 (diff | hist) N Team:Washington/Protocols/gel electrophoresis (Created page with "{{Template:Team:Washington/Templates/Top}} =Agarose Gel Electrophoresis= == General Procedure == #Cast a gel #Place it in gel box in running buffer #Load samples #Run the gel...")
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