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User contributions
From 2012.igem.org
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- 20:03, 19 June 2012 (diff | hist) Team:WashU/DesignSynecho
- 19:55, 19 June 2012 (diff | hist) Team:WashU/DesignSynecho
- 18:21, 19 June 2012 (diff | hist) Team:WashU/Week4 (→E. coli)
- 17:56, 19 June 2012 (diff | hist) Team:WashU/Week4 (→Synechocystis)
- 17:37, 19 June 2012 (diff | hist) Team:WashU/DesignSynecho (→Rising Action)
- 17:36, 19 June 2012 (diff | hist) Team:WashU/DesignSynecho (→Rising Action)
- 16:31, 19 June 2012 (diff | hist) Team:WashU/DesignSynecho
- 15:36, 19 June 2012 (diff | hist) N Team:WashU/PartsSynecho (Created page with "window.onload = function() { ZebraTable.stripe('mytable'); } </script> <style type="text/css"> body { background: #8f0222; } table { border-collapse: collapse; width: 50%; margin...") (top)
- 17:33, 18 June 2012 (diff | hist) Team:WashU/Protocols/6803media
- 17:30, 18 June 2012 (diff | hist) Team:WashU/Protocols/Transformation
- 17:28, 18 June 2012 (diff | hist) Team:WashU/Protocols/gel electrophoresis
- 17:28, 18 June 2012 (diff | hist) Team:WashU/Protocols/Digestion
- 17:28, 18 June 2012 (diff | hist) Team:WashU/Protocols/gel electrophoresis
- 17:27, 18 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 17:25, 18 June 2012 (diff | hist) Team:WashU/Protocols/gel electrophoresis
- 17:13, 18 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 17:12, 18 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 17:12, 18 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 17:12, 18 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 15:25, 15 June 2012 (diff | hist) N Team:WashU/Protocols/6803media (Created page with "{{WashUback}} =Synechocystis 6803 Media= == 1L agar for freshwater cyanobacteria (no glucose) == #Mix 10 g agar and 1 mM thiosulfate (= 0.248 g), top off with H20 to 500 mL to...")
- 15:21, 15 June 2012 (diff | hist) Team:WashU/Protocols
- 21:42, 14 June 2012 (diff | hist) Team:WashU/Week3
- 21:41, 14 June 2012 (diff | hist) Team:WashU/Week3
- 15:57, 14 June 2012 (diff | hist) Team:WashU/Protocols/Transformation6803
- 15:55, 14 June 2012 (diff | hist) Team:WashU/Protocols/Transformation6803
- 15:54, 14 June 2012 (diff | hist) Team:WashU/Protocols/Transformation6803
- 15:54, 14 June 2012 (diff | hist) N Team:WashU/Protocols/Transformation6803 (Created page with "1. Make sure the cells are in exponential growth phase. (OD730 of 0.8) 2. Take 15 ml of cell culture and spin it down for 15 minutes at RT. 3. ReSuspend the pellet to 1ml in BG11...")
- 15:53, 14 June 2012 (diff | hist) Team:WashU/Protocols
- 15:53, 14 June 2012 (diff | hist) Team:WashU/Protocols
- 20:54, 13 June 2012 (diff | hist) N Team:WashU/Protocols/Transformation (Created page with "{{WashUback}} =General Transformation Protocol= #Start thawing the competent cells on ice. #Add 50 µL of thawed competent cells into pre-chilled 2ml tube. #Add 1 - 2 µL of t...")
- 20:52, 13 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 20:51, 13 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 20:49, 13 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 20:47, 13 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 20:46, 13 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 20:45, 13 June 2012 (diff | hist) Team:WashU/Protocols/Ligation
- 20:44, 13 June 2012 (diff | hist) N Team:WashU/Protocols/Ligation (Created page with " =General Ligation Protocol= #Ligate the Upstream and Downstream Parts into the digested Destination Plasmid. Upstream Part digestion: 2 µl Downstream Part digestion: 2 µl ...")
- 20:34, 13 June 2012 (diff | hist) N Team:WashU/Protocols/Digestion (Created page with "{{WashUback}} =General Digestion Protocol= 1.Digest Upstream Part with EcoRI-HF™ and SpeI. Add 500 ng of plasmid DNA, EcoRI-HF (1 µl), Spel (1 µl), 10X NEBuffer 2 (5 µl),...")
- 20:26, 13 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 20:24, 13 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 20:22, 13 June 2012 (diff | hist) Team:WashU/Protocols/PCR
- 20:21, 13 June 2012 (diff | hist) N Team:WashU/Protocols/PCR (Created page with "=General PCR Protocol= #Setup Amplification PCR Reaction ##1uL Template ##1uL 25mM dNTP's ##10uL Phusion HF Buffer ##0.5uL Forward Primer (Tm XX <sup>o</sup>C)* ##0.5uL Reverse ...")
- 19:47, 13 June 2012 (diff | hist) Team:WashU/Protocols/gel electrophoresis
- 17:53, 13 June 2012 (diff | hist) Team:WashU/Protocols/gel electrophoresis
- 17:50, 13 June 2012 (diff | hist) Team:WashU/Protocols/gel electrophoresis
- 17:42, 13 June 2012 (diff | hist) N Team:WashU/Protocols/gel electrophoresis (Created page with "=Agarose Gel Electrophoresis= == General Procedure == #Cast a gel #Place it in gel box in running buffer #Load samples #Run the gel #Image the gel == Casting Gels == '''The ...")
- 17:36, 13 June 2012 (diff | hist) Team:WashU/Protocols
- 17:31, 13 June 2012 (diff | hist) Team:WashU/Protocols
- 17:24, 13 June 2012 (diff | hist) Team:WashU/Protocols
- 20:39, 5 June 2012 (diff | hist) Team:WashU/Team/Tony
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