Tuesday 7/8/12

(Click on headings to visit methods)

Making liquid media, plates & buffers

Liquid LB Media

SOC Solution

SOB Solution

LB Agar Plates

Ampicillin LB Agar Plates: 31 plates

Chloramphenicol LB Agar Plates: 33 Plates

Kanamyacin LB Agar Plates: 32 Plates

TB buffer.

TAE buffer.

EDTA buffer.

Designing Gibson Assembly Fragments

Haemoxygenase Gene
Deinococcus radiodurans Bacteriophytochrome Gene
Agrobacterium tumefaciens Bacteriophytochrome Gene

Tuesday 14/8/12

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Making Competent Cells

Choosing Biobricks
Making competent cells
Transformations

Outreach Planning Labwork

Open Day

Secondary Education Seminars

Wednesday 15/8/12

Our open day preparation did not go as planned. Most of our transformations were unsuccessful, we believe this was because SOC was added after the heat shock. As disappointing as this was, there was one little piece of good news.

A single colony of Top 10 E.coli containing GFP insert was identified on a plate of LB agar media.

LB agar and nutrient agar plates were struck using this colony. The colony was also used to inoculate 5mL of nutrient broth. Plates and stock were then incubated at 37°C overnight. Both of the streaked plates and the broth showed significant growth of E.coli colonies which could fluoresce when exposed to UV light.

Unfortunately, Qantas declined to sponsor the effort of getting our team to Hong Kong. This was soon forgotten when the Macquarie Faculty of Medicine, Agilent and the Defence Science Institute (thanks Yagiz) both agreed to sponsor our team. Hong Kong here we come.