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Team:TU Darmstadt/Protocols
From 2012.igem.org
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- In-fusion BioBrick assembly
- Miniprep / Qiagen kit
- Transformation of chemically competent cells
- Western Blot
Comamonas testosteroni
In vitro
Nucleic acids
DNA
- Agarose gel electrohporesis
- Annealing and primer extension
- Annealing primers
- Assembly PCR
- Cloning
- DNA Ligation
- DNA Quantification / NanoDrop
- DNA Synthesis from Oligos
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Miniprep
- PCR
- Polyacrylamide gel electrophoresis
- Primer Design
- Purification of DNA
- Restriction digest
- Resuspension of primers
- Salting Out
- Sequencing DNA
RNA
Protein
In silico
Data analysis
Databases
Modelling
Sequence analysis
Structure analysis
Miscellaneous
Cell culture
Microscopy
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
