Team:Paris Bettencourt/Semantic containment

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Overview

We need to prevent our genetic construction from being used by other organism. Since horizontal gene transfer (HGT) can be perform either by conjugation, by transduction, or by transformation, and none of these systems is only dependent of our organism, we cannot assume the fact that HGT is fully avoidable. Semantic containment [1] means that our bacteria won't be able to "speak" with other organism, since they don't speak the same language. The language being DNA. Here, it won't be all DNA that we are going to change, but just 1 stop codon that we are going to change in a normal amino-acid codon in our synthetic bacteria. It means that in our bacteria the stop codon will be translate into an amino-acids, whereas in wild type bacteria, the gene won't be translate, and the protein won't be efficient.

Objectives

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Design

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Experiments and results

Characterisation of X

Experimental setup

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Results

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Testing of the system

Experimental setup

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Results

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