Team:Macquarie Australia/Protocols/ArrivalofGBlocks

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Revision as of 05:17, 31 August 2012 by Ehoyer (Talk | contribs)



Ordered gBlock fragments are nearly here!

Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks:

  • +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
  • -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
  • +T7 promoter Heme oxygenase
  • -T7 promoter Heme oxygenase

Click on each above to see fragments which will be assembled using Gibson and fragment properties.


Biobrick Name Part(s)
T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B
Heme oxygenase (T7 minus) Plasmid Hemo_A Hemo_B
T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E
Agro (T7 minus) Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E
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