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From 2012.igem.org

Contents 1 Tuesday 7/8/12 1.1 Making liquid media, plates & buffers 1.2 Designing Gibson Assembly Fragments 2 Tuesday 14/8/12 2.1 Making Competent Cells 2.2 Outreach Planning Labwork 3 Thursday 16/8/12

Tuesday 7/8/12

(Click on headings to visit methods)

Making liquid media, plates & buffers

• Liquid LB Media

• SOC Solution

• SOB Solution

• LB Agar Plates

Ampicillin LB Agar Plates: 31 plates

Chloramphenicol LB Agar Plates: 33 Plates

Kanamyacin LB Agar Plates: 32 Plates

• TB buffer.

• TAE buffer.

• EDTA buffer.

Designing Gibson Assembly Fragments

• Haemoxygenase Gene
• Deinococcus radiodurans Bacteriophytochrome Gene
• Agrobacterium tumefaciens Bacteriophytochrome Gene

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Tuesday 14/8/12

(Click on headings to visit methods)

Making Competent Cells

• Choosing Biobricks
• Making competent cells
• Transformations

Outreach Planning Labwork

• Open Day

• Secondary Education Seminars

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Thursday 16/8/12

Our open day preparation did not originally go as planned. Of all our constructs (Green, orange, cyan, red fluorescent protein) only one GFP colony was grown. This was subcultured onto nutrient agar and ampicilin, a broth was also innoculated. This was observed under UV-light- it was fluorescent! We decided to continue with this inoculate.