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During the creation

Contents 1 Notebook 1.1 8/9 1.2 8/11 1.2.1 The purified DNA1,2 1.2.2 PCR Product 1,3,9

Notebook

8/9

8/11

The purified DNA1,2

1. Electrophoresis
   • Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL
   • Sample:pigment(buffer) 1μL,sample 5μL
   • Gel concentration:1.2%,Migration time:30minutes
2. Storage

PCR Product 1,3,9

1. Electrophoresis
   • The gel check and cut
2. DNA purification
3. Confirmation of electrophoresis
4. PCR
   • 50cycle
5. Storage

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