Since the fermentation industry occurred, the phage infection has become a big threat to engineering bacteria. During mass production of dairy products, enzymes, amino acids, antibiotics and pesticides, the results are unimaginable if the bacterias are infected by bacteriophages . The bacterias will die abnormally and the infection will spread quickly. Without any efficient action, tremendous loss will lie ahead. Nowadays, common strategies for avoiding this catastrophe is to control the conditions of the fermentation strictly. Also, people keep selecting strains against phages. But all the strategies are time consuming and far from once for all.
However, the engineering bacteria 2012 USTC-China iGEMers hope to cultivate both protecting themselves when bacteriophage invades the strain and focusing on producing products without consuming extra energy when their living environment is safe. In this case, the quality of fermented products can be guaranteed and the profits of fermentation industry can be enormously improved.
A nonlethal concentration of H202(0.05%) greatly enhances near-ultraviolet(NUV) inactivation of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected into the bacterial host, but not-the number of phage adsorbed.Not only were recombination and gene expression of late markers reduced up on treatment of phage'11withNUVplusH202,but also a gradient of recombination resulted,with markers injected first reduced to a lesser extent than those injected last.Double-strand DNA breaks were not detected
A nonlethal concentration of H202(0.05%) greatly enhances near-ultraviolet(NUV) inactivation of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected into the bacterial host, but not-the number of phage adsorbed.Not only were recombination and gene expression of late markers reduced up on treatment of phage'11withNUVplusH202,but also a gradient of recombination resulted,with markers injected first reduced to a lesser extent than those injected last.Double-strand DNA breaks were not detected
A nonlethal concentration of H202(0.05%) greatly enhances near-ultraviolet(NUV) inactivation of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected into the bacterial host, but not-the number of phage adsorbed.Not only were recombination and gene expression of late markers reduced up on treatment of phage'11withNUVplusH202,but also a gradient of recombination resulted,with markers injected first reduced to a lesser extent than those injected last.Double-strand DNA breaks were not detected
What do we want to do
This year, USTC-China iGEMers are providing a novel immune system for engineering bacteria to fight against the bacteriophage. We hope this artificial line of defeat with gene circuits can be of practical use in fermentation industry.
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