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From 2012.igem.org

Monday: Well, today was our first group meeting after the final exams so yes, most of us seemed to be taken from a "The Walking Dead" set, but still none of us fell asleep (great achievement team!). Emi couldn't make it physically because she's still recovering from mononucleosis but she was present thanks to google's Hang Out. During the meeting... Basically we defined guidelines to follow and tasks to complete before the wiki freeze. The next two weeks were planned according to who is going to leave for holidays (Simon decided to spend all is money in Las Vegas, Carla is going to drink as much beer as she can at her homeland, Max will be off searching for white walkers way beyond the wall) and the rest of the slave... I mean team members that will be staying in Santiago therefore in the lab. So, Simon will transfer all of his wet lab knowledge to Isaac and Tama, meaning they are going to be in charge of the ligations and restrictions for the next three weeks. Max will be reading dusty books and ancient rotten scrolls (or the newest issues of Science, Nature and others might also help) in order to find a new protein secretion system for spider silk. He'll be replaced by Ulises starting from July 23. All arduino related work will be under Carla's command, once she leaves Arduino will be in stand by (just for one week only). Bryon needs to get results in his other lab ASAP so we won't see him around much. Seba will be studying for his big B.S. degree exam so he is in the same status as Bryon (not around much). Claudia is finishing her M.S. thesis so... I really don't know. Emi, emi emi... please get better soon!!! She'll be doing as much work as she can while in bed, most will be wiki material. And that leaves us Pollak... :) lab work 24x7. I just hope you get to eat something. THE ARDUINO QUEST Arduino is here :)

Tuesday: Doing our first (failed) attempts with arduino

Wednesday:



Thursday: this is the day crazyengineer42 (meaning me) took the day off and wandered all day long through the streets of Santiago trying to find his/herself.

Meanwhile in the lab: Did a PCR colony protocol to check if psbAB-sfGFP was inserted in pPMQAK. Also, did a vector backbone PCR for psb4k5 and psb1C3.

Fail: wrong plasmid templates added to colony PCR. This will probably lead to dimer formation.

Friday: We have our first Arduino project running :D It's a temperature sensor that sends the message "Pollak screw u :)" to the program console if the temperature is between 20 - 30 °C. That's the twisted sort of way we demostrate our gratitude feelings towards our instructor. Also, every time the temperature drops, the message "Brace yourselves, winter is coming" is sent. (The arduino can turn off and on a LED as well but that's not a big deal!).

Wet lab: PCR colony indeed failed and the other backbones didn't turn ok. The experiment was repeated.