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From 2012.igem.org

Monday: Arduino is here :)

Tuesday: Doing our first (failed) attempts with arduino

Wednesday:



Thursday: this is the day crazyengineer42 (meaning me) took the day off and wandered all day long through the streets of Santiago trying to find his/herself.

Meanwhile in the lab: Did a PCR colony protocol to check if psbAB-sfGFP was inserted in pPMQAK. Also, did a vector backbone PCR for psb4k5 and psb1C3.

Fail: wrong plasmid templates added to colony PCR. This will probably lead to dimer formation.

Friday: We have our first Arduino project running :D It's a temperature sensor that sends the message "Pollak screw u :)" to the program console if the temperature is between 20 - 30 °C. That's the twisted sort of way we demostrate our gratitude feelings towards our instructor. Also, every time the temperature drops, the message "Brace yourselves, winter is coming" is sent. (The arduino can turn off and on a LED as well but that's not a big deal!).

Wet lab: PCR colony indeed failed and the other backbones didn't turn ok. The experiment was repeated.