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Team:St Andrews/Procedure
From 2012.igem.org
Lab Book
Procedure
anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.
Please also refer to our Protocols page.
Start from lipid extraction
primers, sequence results
Δ12 T. Cruzi
atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtg
cttaagtctgggcgcaacaaggagcacaatgtcattgtgccttcctccgcactgaccatt
ggagaaattcaggagaaaatcccggtaaagtactttgaacgcaacactacccggagcgtc
atgtttcttttacgcgacctcgcacaggtggcgttgacgtacgccattatgtacgccgtc
gccttgccacttgccacttccatggaagtttctgcagcaagaacgttggccgacggtggg
gtgttatcattgatggcaggtacagccatgacgacagcggcgtggcttttaaaggcagtg
ttgtgggcggtgttttggtttgtacagggacttaacggcactgcgctttgggtcctggct
cacgaatgcggccatcaggcgttcagccccatgaaaggtttgaatgacgccgttggcatg
attttgcactctgcgctgctcgtgccgtaccacagctggcgcatcacccacggcggccac
cacaaacacacgaatcacctcacgaaggatcttgtgtttgttccagacaaacgaaacgcg
gttgtggagctcgtggaggaggcgccgctggtgttattaattcaattattgctgattttt
ctctttggttggccggcacatcttctttttaatgcctctggacaggaatttggccgactc
gcgagccactttgaccccggcgctccatttttccgcagcgaagaccgtcacgatattgtc
ctgtcgaatgttgggattgtcagcgcgttatttgtcattttctccagcgtttaccgcttt
ggttttacaaatgttttctgctggtacattgtaccgtacctctgggtgaacttttggctt
gtgtacattacatacctgcagcacacggatatacgcattcctcactacacacatgagcac
tggacgtttgttcgcggtgcattggcggctgtggacagggactacggctttgtccttaac
acatggctccatcacatcaatgattcccacgtggtacatcacctctttagcaaaatgcca
cactacaacgcaatccaggtgacaagaaagtacattcgtgagatactgggtgccacatac
attacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctat
gttgttcccgcagagggcgtctgtgtctttcatgggtaa
Δ12 Synechocystis
atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
aaagcgagcaaagcctgggcttctgttttgattaccctaggggcgatcgccgtgggctat
ttgggcattatttatctgccctggtactgcttgcccattacctggatctggacagggaca
gccttaacgggggccttcgttgtcggccatgactgtggccatcgctcctttgctaaaaaa
cgctgggtcaatgatttagtgggacatatcgcttttgctcccctcatctaccctttccat
agctggcgcctactccacgaccaccatcacctccacaccaacaaaattgaggttgataac
gcctgggatccctggagtgtggaagctttccaagccagcccggcgatcgtccggcttttt
tatcgggccatccggggtcccttctggtggactggttccattttccattggagcttaatg
cacttcaaactttccaactttgcccaaagggaccgcaataaagtcaaattatccattgcc
gttgtcttcctgtttgcggcgatcgcctttcctgccctaattatcaccacaggggtgtgg
ggtttcgtcaaattttggctaatgccctggttggtgtatcacttttggatgagcactttt
accattgtgcaccacaccattcccgaaattcgtttccgtcccgccgccgattggagtgcc
gccgaagcccagttaaatggtactgttcactgcgattatccccgttgggtggaagtgctc
tgccatgacatcaacgtccatattccccaccacctctccgttgccatcccttcctataac
ctacgactagcccacggaagtttaaaagaaaactggggaccttttctttacgagcgcacc
tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
ggctaccgcaccttcggctccctgaaaaaagtttaa
Δ15 Synechocystis
gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
gtccggtccttgggctacttttttttggatgttggtttaattgccgggttttatgctcta
gcggcctaccttgattcctggttcttctatccgattttttggttaattcagggaacccta
ttctggtccctgtttgtggtgggccatgattgtggccatggctccttttccaaatccaaa
acccttaataattggattggtcatctcagccacacgccaattttggtgccttaccatggc
tggcgtattagtcatcgtactcaccatgccaacacgggcaatatcgacaccgacgaaagt
tggtatccagtgtcggagcaaaaatataaccaaatggcctggtatgaaaaacttctacgt
ttttacttgcctctgatcgcctaccccatttatctatttcggcgatcgccaaaccggcaa
ggctcccatttcatgcccggcagtcccctattccgtcccggagaaaaagcagctgttctc
accagcacctttgcccttgcagcctttgtcggcttccttggctttttaacttggcaattt
ggctggctatttttgctgaaattttatgttgccccctacctcgtgtttgtggtgtggtta
gatttggtcacatttttacatcacactgaagacaatatcccttggtatcgtggtgatgac
tggtattttctcaaaggtgccctctccaccattgatcgggattacggcttcattaacccc
attcaccatgacattggcacccacgtcgcccaccatattttctcgaatatgccccactac
aagttacgccgggcgactgaagccatcaagcccattttaggggaatattatcgatattct
gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa
Δ6 Synechocystis
atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
tatctgaaaaccctgattattgtgctctggttgttttccgcttgggcctttgtgcttttt
gctccagttatttttccggtgcgcctactgggttgtatggttttggcgatcgccttggcg
gccttttccttcaatgtcggccacgatgccaaccacaatgcctattcctccaatccccac
atcaaccgggttctgggcatgacctacgattttgtcgggttatctagttttctttggcgc
tatcgccacaactatttgcaccacacctacaccaatattcttggccatgacgtggaaatc
catggagatggcgcagtacgtatgagtcctgaacaagaacatgttggtatttatcgtttc
cagcaattttatatttggggtttatatcttttcattcccttttattggtttctctacgat
gtctacctagtgcttaataaaggcaaatatcacgaccataaaattcctcctttccagccc
ctagaattagctagtttgctagggattaagctattatggctcggctacgttttcggctta
cctctggctctgggcttttccattcctgaagtattaattggtgcttcggtaacctatatg
acctatggcatcgtggtttgcaccatctttatgctggcccatgtgttggaatcaactgaa
tttctcacccccgatggtgaatccggtgccattgatgacgagtgggctatttgccaaatt
cgtaccacggccaattttgccaccaataatcccttttggaactggttttgtggcggttta
aatcaccaagttacccaccatcttttccccaatatttgtcatattcactatccccaattg
gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga
ELO 6 L. major
atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
cgcccgaacctcattactctgtcagataaggtgcccctgtcgcagcagggggtgaaggat
ggctcggtgatcacttacaaggacctcggcccgcagatcggctaccgcacggtgttctac
gtcgagtatgccggccccatcgccttcatgctgctgtacgccatgcgcccttcgctcatc
tacggctctgccccgatgccggcttacggctacacgcagaagctatacattggcctcttc
ctcgcccacttcttaaagcgcgagctcgagtccatgtttgtgcacaagttctcgcaccca
acgatgccgatgcgcaacatcttcaagaactgcatctactactggtccttcgccgccttc
atcggctacgtgctgtgcagtccttcattcacgccgaccagcaccatgcagtcaaacttc
ggcgccgtggtcatggtcatcaacgagctgctgaacttcgcggtgcactaccagcttagc
acgatgcgcaagtccgatggtgacaccacccgcaacgtgccgcaaggccctctgttcgcc
ttcgtctcgtgcccgaactacttctttgagattatgtcgtgggtgtccttttccatcggc
acaaatatgttatcctcctggttcttcacactcgccggtttcgtgcagatggcggactgg
gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
attctgcccttcatcatgtag
- date
- what we did
who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely.
Retrieved from "http://2012.igem.org/Team:St_Andrews/Procedure"
