"

From 2012.igem.org

Home	Team	Project	Parts	Modeling	Notebook	Protocols	Safety	Attributions

Contents 1 Protocols 1.1 DNA Extraction 1.2 DNA Assessment 1.3 PCR Methods 1.4 Plasmid Insertion 1.5 Plasmid Editing 1.6 Other 1.7 Maintenance Tasks

Protocols

DNA Extraction

• Miniprep
• Gel Purification

DNA Assessment

• Gel Electrophoresis
• DNA Quantification
• Sequencing

PCR Methods

• Vent PCR
• Phusion PCR
• Colony PCR

Plasmid Insertion

• Transformation of E. coli
• Transformation of Shewanella
• Conjugation

Plasmid Editing

• Digestion Reaction
• Ligation Reaction
• Gibson Assembly

Other

• Dephosphorylation
• PCR Clean Up

Maintenance Tasks

• Making LB
• Autoclave
• Making Super Optimal Broths
• Preparing Glycerol Stocks
• Preparing Overnight Cultures