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Team:Tec-Monterrey/antifreeze/project
From 2012.igem.org
This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing
and thawing, due to the expression of antifreeze proteins from the beetle Rhagium inquisitor (RiAFP). We expect this
new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.
We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the necessary
sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will
use an experimental design to find out the best storing conditions for this strain during cryopreservation.
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This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells. We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the necessary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation. |
